A hospital supported by the aid group has received "dozens of dead and wounded", adding that "under global humanitarian law, civilians must be protected during conflict".
"US military support to our partners mitigates noncombatant casualties", said Pentagon spokeswoman Rebecca Rebarich, according to the statement.
There was no immediate comment from the coalition, which intervened in 2015 to try to restore the internationally recognized government to power after it was driven out of the capital Sanaa by the rebels.
Airstrike by U.S. ally on bus carrying Yemeni children kills and wounds scores
The Western-backed alliance fighting the Iranian-aligned Houthi group in Yemen accused the Houthis of using children as human shields.
He said the leaders and militants responsible for launching ballistic missiles and targeting civilians would be held accountable as part of the coalition's efforts to prevent terrorist elements from compromising regional and global security.
In 2014, the Houthis overran the Yemeni capital and seized control of much of northern Yemen as well as a string of ports on the Red Sea.
It was unclear how many children were killed and how many air strikes were carried out in the area, in northern Yemen, near the border with Saudi Arabia.
Yemen has been devastated by a conflict that escalated in early 2015, when the Houthis seized control of much of the west of the country and forced President Abdrabbuh Mansour Hadi to flee overseas.
"We trust when they say that they will investigate that they will investigate".
Meanwhile, elite pro-government forces in Shabwa launched an operation on Wednesday to disarm residents in the south-eastern province.
Media captionThe conflict in Yemen has been raging for years - but what is it all about? They comprise mostly AK-47s and small arms, but in some cases authorities have found heavy machine guns and bombs, in particular since Al Qaeda established a presence in the country in 2011. It was not immediately clear if there were any casualties in those strikes.
Persied meteor shower peaks this weekend
The Perseid meteor shower may be an annual event, but it's such an exciting celestial show that it's always worth another look. As meteors enter the earth's atmosphere they leave streaks of light in the sky, which some people call shooting stars.
Global rights groups repeated calls for an end to the war and strongly condemned the bus attack and other civilian massacres.
The first day of the campaign rounded up 500 weapons from tribal members in the area, said Col Al Buhar. Residents remain apprehensive of disarmament because of lack of confidence in the authorities.
Impoverished Yemen, on the southern tip of the Arabian Peninsula, is now in the world's worst humanitarian crisis, with more than 22.2 million people in need of assistance.
Earlier this week, the UN Special Envoy for Yemen, Martin Griffiths, convened a consultative meeting in the United Kingdom of 22 Yemeni public figures and women activists as part of his efforts to end the war.
Margot Kidder's Death Ruled Suicidal Overdose
She was also a political activist who was arrested in Washington in 2011 at a protest over the proposed Keystone XL pipeline. As it was previously reported, the police discovered Margot passed away on the 13th of May in her home in Livingston.
Red Dead Redemption 2's first gameplay footage
We catch glimpses of mountains, dense forests, steamy swamps, deserts, dusty cattle towns, more modern cities, and more. A narrator goes over everything throughout the video, explaining important information about what people are seeing.
Tribune Media scraps Sinclair deal
The American Cable Association (ACA), which represents small- and medium-sized cable companies, also cheered Tribune's decision. Tribune Media would be on the hook for a $135 million breakup fee, according to the agreement reached a year ago .
In Women's Heart Attacks, a Doctor's Gender Matters
Women doctors, in 2017, earned 27.7 percent less than their male counterparts, an average of $105,000 less a year. But when female physicians took charge of treatment, those percentages fell - to 11.8% of men and 12% of women .
Israel PM defends law in face of Druze outrage
The wave of criticism piles pressure on Prime Minister Benjamin Netanyahu, already beset by corruption allegations. It is at least the thirds challenge filed with the Supreme Court over the law.
The Oscars Are Changing Because You Aren’t Watching
But Wednesday's announcement means there are more opportunities for those films to be recognized during Hollywood's Biggest Night. The next Oscars ceremony will take place in Hollywood on February 24, as previously announced.